LOCUS       40924_21529        6216 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGFcyp1-hph DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Sato M, Kurahashi A, Nishibori K, Fujimori F.
  TITLE     Development of a transformation system for the edible mushroom 
            Grifola frondosa: demonstrating heterologous gene expression and 
            RNAi-mediated gene silencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Sato M, Kurahashi A, Nishibori K, Fujimori F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2014) Contact:Masayuki Sato Yukiguni Maitake Co. 
            Ltd., Research and Development; 89 Yokawa, Minamiuonuma, Niigata 
            949-6695, Japan URL :http://www.maitake.co.jp
REFERENCE   3  (bases 1 to 6216)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6216)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-FEB-2014) Contact:Masayuki Sato Yukiguni Maitake Co. Ltd., 
            Research and Development; 89 Yokawa, Minamiuonuma, Niigata 949-6695,
            Japan URL :http://www.maitake.co.jp"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..1500
                     /gene="Gf.CYP1"
                     /regulatory_class="promoter"
     gene            1..1500
                     /gene="Gf.CYP1"
                     /label=Gf.CYP1
     CDS             1501..2523
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      2527..3526
                     /gene="Gf.CYP1"
                     /regulatory_class="terminator"
     gene            2527..3526
                     /gene="Gf.CYP1"
                     /label=Gf.CYP1
     primer_bind     complement(3574..3590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3598..3614)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3622..3652)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3667..3688)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3976..4564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4738..5595)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5596..5700)
                     /label=AmpR promoter
     primer_bind     6174..6190
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atcaacgcca taggcacacc ctacaggata tgcaccgccg tcatatagaa atagccggag
       61 gtggcgtcag acagggtagc gatataaccg gtagggctcg aagggatcgc caatttagag
      121 gctagcatgc accaggacgt gagcgagaga ttccaacgaa aaggctcgac catattcgca
      181 ggttagcgaa gggtggcaga tggacggatt ctgttatcag ggccaaacct tcatcgcaga
      241 aatttcaaaa atgtgccagc caaccgtgac ggaaagcatc gttcttgaga gatattcgct
      301 caagaagacg gtttcaggaa accgggacaa cttcagctcg ggagattcat tcggtgtgag
      361 tgtggatacg ctgagctcaa agcgcgtaca gttcggaaca gatgtctgga aacgagctgt
      421 cagattgtaa ggaattttaa agatggaggt ggatgacaaa tagagcgcac cgcgcgagtt
      481 atggccacgc cgggaacgga agaagcggag aagtaaggaa gacacagctc atatgttatg
      541 taacgagtgg atacagcggg cgcgaactac gcgttctcac cataccgcgg gaaaggaaaa
      601 ggaatggatg cacgcacaca attggagtaa tcaatgatca agtgggagcg ctcgagcgag
      661 ttgcgagtgc tggttatgac gctatgagcc gtgcttgccg gactcattgt tggcaagtct
      721 cctgggagcg aaaaagttcc attgacagcg tgaagagcta acaataatac gtagactcaa
      781 cagaggaaga cgccgacaga ctatttgaag ggtcggtcgg aaaaggcata aatctccacg
      841 accggcgcga ccagaacgca catgacgctc aaaataaagg caactaggga ttcgcaaaat
      901 taaggaccgc gtttcagctg tgtctggcga acactgactt cagttctcgt cacttgccca
      961 aaatcgtatt ctagttactc acaatatacc ggtgtcgacg aacaccgagt ggtctaattg
     1021 gccggaagag cggggatttc gactgtcgga gcatagagaa gaaagcactg agagggaata
     1081 ttcactgtgt atgagccaat ttctccgttt gcgattccaa aattcgagtc gacccggact
     1141 gggccttcgg agggagcgtc cgtttgtccg aggatacata acggggtgcg cgctgtcctc
     1201 aaaagcacag actacgaatg attctgagac tatggcatgc ctctgggata tacctttgga
     1261 gaattgtggc caagaatgtc cgacggaagt ccaacgttgc gaacatcaag aatattccat
     1321 tggcagttca gacaaaataa gaaaatgtca cagatggtga cagattgtca caagcggtta
     1381 gataatcatc agcgtgattt tatagctgat cacctccgat cggcagttgc cgcgctgctt
     1441 taagagccca gctcccctgc gcaccacaga atcacttccc ctttccttca cgccttcata
     1501 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     1561 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     1621 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     1681 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     1741 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     1801 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     1861 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     1921 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     1981 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     2041 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     2101 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     2161 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     2221 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     2281 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     2341 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     2401 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     2461 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     2521 gaatagaacg ttgtttgtta tgtctgaagg tgtggttgag gccattacgg acttgttggg
     2581 gttgcggctc agaagcagaa gcagaagata atccaccctg tactctcatc gacccacatt
     2641 gcacaagtgt tgtctatgtg ataatagatg gtctgacttg tatgatcatc aaggtgttgt
     2701 gcgcgttcgg ttcacatgac gatatcagtt gtcatcgaac gttttcccct ggctatgctg
     2761 aatggagcga ctatgcatcg tcgtccatgt acgttgcgag tatcaggtgc aaccactaga
     2821 agcaagctcc ttgtgacctg aaggaagtcc tttaagacac ttaaagaact cgaggtcatg
     2881 cgtgcgcttt caggatggag tatcaagttt agaaacatcg gagttgcgcg cacgttcgag
     2941 atagtagcgc atggatatca tcacgtcaga tggacagcat taagaagacc gtggcgtacc
     3001 cattcgacat cgaaaatagg tggtgaatat ctgacgccgc cgtggtatgc atgcctaccc
     3061 ttgtcgatat acggaaacac cagcgaatat atcaatggat tattcattct aacctccaat
     3121 acgtaagagc gaagatgtgc tctgtgccaa gcgccatcgg acgaagtcaa gagaattata
     3181 ctcaatactt agtcggcatc aggataccta gtcgacctcg agacaaatac agcgcataca
     3241 tctgctacgt taacgttggg tccgcagaag tagcctcttt tggaatgtcg gcttccccat
     3301 atggacgtgt gaagctagtc gccatcgttg cgcaagatgg cacagacttt ctcacagacg
     3361 tccaacaatc tattctcagg acgatagcat tcgtaatcga ccgtgatcgc ccaccagtcc
     3421 actctagccc tgcgtcgtcg gttgtgaata tgttgttctc ttccaacacg gcgacctgaa
     3481 cgctattggc cctgaacgac tcggagatcc tgcgttgatc catagagatc ctctagagtc
     3541 gacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     3601 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     3661 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3721 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3781 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3841 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3901 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3961 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4021 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4081 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4141 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4201 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4261 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4321 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4381 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4441 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4501 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4561 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4621 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4681 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4741 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4801 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4861 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4921 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4981 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5041 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5101 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5161 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5221 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5281 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5341 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5401 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5461 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5521 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5581 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5641 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5701 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     5761 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     5821 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     5881 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     5941 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     6001 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     6061 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     6121 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     6181 gacggccagt gaattcgagc tcggtacccg gggatc
//
